COMPARATIVE INVESTIGATION OF ANTIGENS ASSOCIATED WITH PLASMATIC MEMBRANES OF RAT HEPATOMA AND MYOGENIC CELLS
USING ANTI-KIDNEY SERUM
N. P. Teryukova, A. S. Desheva, G. I. Blinova, O. A. Mirgorodskaya, V. A. Ivanov
Institute of Cytology RAS, St. Petersburg;
e-mail: iva@mail.cytspb.rssi.ru
The investigation of antigenic diversion of tumor cells resulting from the expression of heteroorganic antigens
has been continued. Tumor-associated heteroorganic antigens with mol. weight 200—210 kDa (identified
before as laminin), 105—130, 75—80 and 43 kDa were detected by anti-kidney serum in fractions of plasmatic
membranes of cells of rat ascitic Zajdela hepatoma and cultured HTC hepatoma; the antigen 43 kDa was isolated
on immunosorbent and identified by mass spectrometry as β-actin. Anti-kidney serum revealed laminin in
fractions of plasmatic membranes of cultured L8 and L6J1 myoblasts, and L6J1 myotubes; apparently, synthesis
of laminin by hepatoma and myogenic cells is not connected with their proliferative activity. Besides, anti-kidney
serum detected components 38, 42, 44, 48, 62, 78 and 120 kDa, expression of which on myogenic cells surface
might be consequence of active cell proliferation and (or) differentiation.
Key words: β-actin, antigenic diversion, hepatoma, laminin, mass-spectrometry, myogenic cells, tumor-
associated antigens
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