CELLULAR LAMININ-BINDING PROTEIN AND VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS REPLICATION IN VERO, 293
AND 9S2 CELLS
M. M. Gridina, E. V. Protopopova, A. V. Kachko, A. V. Ivanova, E. I. Bondarenko,
V. B. Loktev 1
State Research Center of Virology and Biotechnology «Vector», Koltsovo, Novosibirsk region, 630559;
1 e-mail: loktev@vector.nsc.ru
The level of laminin-binding protein (LBP) expression on cellular membranes was studied in three cell lines
including 293 cells transformed by plasmide with human LBP gene. Vero cells show a high level of LBP on
the cell surfaces and demonstrate a high level of the Venezuelan equine encephalomyelitis (VEE) virus replication.
The inhibition of VEE virus replication was more than 200 times as much after treatment of Vero cell surfaces
with monoclonal antibodies to human LBP. 293 cells have more low level of LBP on their surfaces but being
transformed by plasmide with LBP human gene these cells showed an increase in the level of cellular LBP. The
VEE virus replication in transformed cells (9S2) was more than 2000 times higher compared to 293 cells. The
results obtained demonstrate a principal role of cellular LBP in VEE virus entry into mammalian cells. It can be
proposed that LBP is a key cellular protein at the early stage of VEE virus replication in cells. So, LBP might be
a target protein for development of some new generation of antiviral drugs that would be able to inhibit (enhance)
the alphavirus replication in human cells.
Key words: laminin-binding protein, 293 and Vero cell lines, transformed cells, inhibition of
replication, Venezuelan equine encephalomyelitis virus
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