THE INFLUENCE OF HYDROGEN PEROXIDE ON ABILITY OF NEUTROPHILS TO GENERATE THE REACTIVE OXYGEN AND CHLORINE
SPECIES AND SECRETE MYELOPEROXIDASE IN VITRO
E. I. Kovalenko,1 G. N. Semenkova, S. N. Cherenkevich
Biophysics Department of Physical Faculty of Belarusian State University, Minsk;
1 e-mail: kavalenka@bsu.by
The influence of Í2Î2 at concentrations of 10-8-10-2 mol/l on
neutrophil ability to generate the reactive oxygen and chlorine species (ROCS) and secrete myeloperoxidase (MPO) was
studied, and Í2Î2 injurious effect on neutrophils was also investigated in this work. It was
revealed that Í2Î2 at concentrations of 2 • 10-3-2 • 10-2
mol/l induced disturbance of the neutrophil membrane barrier properties and lactate dehydrogenase release. The
incubation of the neutrophils with the addition of 10-4-10-7 mol/l Í2Î2
led to an increase in the cell ability to generate ROCS during phagocytosis and decreased neutrophil ability to
secrete MPO and ROCS in extracellular medium during adhesion. The mechanisms of Í2Î2 effect are
coupled with arachidonic acid metabolism. Inhibition of metabolic pathways of 5-lipoxygenase or cyclooxygenase
increased the destructive effect of Í2Î2 on the cells. Five-lipoxygenase way prohibition led to
cancellation of Í2Î2 influence on MPO and ROCS secretion and to enhancement of
Í2Î2 effect on neutrophil ability to generate ROCS during phagocytosis. The data obtained
testify to the high neutrophil resistance to destructive effect of Í2Î2 and confirm the
regulatory role of Í2Î2 with respect to the neutrophil functions.
Key words: neutrophils, hydrogen peroxide, reactive oxygen and chlorine species, myeloperoxidase,
5-lipoxygenase, cyclooxygenase, lactate dehydrogenase
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