CYTOLOGICAL STUDY OF AREA OF ISCHEMIC ALTERATION IN RAT KIDNEY
M. Aunapuu,1* P. Roosaar,1 T. Suuroja,2 A. Arend 1
1 1Department of Anatomy, University of Tartu, Ravila 19, 51014 Tartu, Estonia,
and 2 Department of Histology, Estonian Agricultural University, Kreutzwaldi 64, 51014 Tartu, Estonia;
* e-mail: mariaun@ut.ee
The course of reparative regeneration after 5/6 nephrectomy and use of low-dose radiation has been studied by
means of light and electron microscopy. The experiments were performed on 30 male Wistar rats. All animal procedures
were conducted after approval of the protocol by the animal Studies Ethics Committee of the University of Tartu.
Renal ablation was then accomplished by right nephrectomy and selective ligation of extrarenal branches of the left
renal artery such that approximately 2/3 of the left kidney was infracted. All together 30 rats were randomised after
the surgery and divided into two groups matched for age and body weight at week 0 and studied during 2, 4 and 8 weeks:
groups I (nephrectomized, n = 15), groups II (nephrectomized and irradiated, n = 15). Left kidney of II groups rats
was irradiated (60Co) 24 h after surgery in anaesthetized (Brietal) animals with 3 Gy in a single dose.
As a result of experimentally induced ischemia destruction of renal corpuscles, perishing of tubular epithelial cells
and and proliferation of connective tissue is followed. Reparative regeneration is based on aseptic inflammation,
duration of its phases depends on the extent of organ impairment. In nephrectomized rats parallel to reparative
regeneration, necrosis and deposition of calcium is found in the cortical substance. Calcium plays important role in
kidney metabolism and its increased content is characteristic to degenerative changes. The experiments reveal that use
of low-dose radiation does not accelerate process of reparative regeneration in rat kidney.
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