CULTURED SKIN CELLS INTERACTION WITH POLYLACTIDE SURFACE COATED BY DIFFERENT COLLAGEN STRUCTURES
Yu. A. Shved,1,* L. V. Kukhareva,2 I. M. Zorin,1 M. I. Blinova,2
A. Yu. Bilibin,1 G. P. Pinaev 2
1 Chemical faculty of St. Petersburg State University, and
2 Institute of Cytology RAS, St. Petersburg;
* e-mail: ulychka@mail.ru
The purpose of this work was an optimization of polylactide film surfaces designed for human keratinocytes
cultivation. The polylactide films were coated by collagen 1. The experiments showed that uniform covering of polymer
surface by collagen, and formation of different collagen structures depend on the mode of the protein application.
The differences in collagen distribution on the polymer surface influened the keratinocytes growth in culture.
Analysis of keratinocytes alignment, as well as cytoskeleton organization demonstrated that fibrillar collagen
promoted more even keratinocytes distribution in comparison with the distribution on molecular collagen.
Key words: cell therapy, biodegradable polymers, polylactide, fibrillar and molecular collagen,
keratinocytes, actin cytoskeleton
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