HETEROGENEITY OF STROMAL PRECURSOR CELLS ISOLATED FROM RAT BONE MARROW
E. B. Anokhina, L. B. Buravkova
SSC RF Institute of Biomedical Problems RAS, Moscow;
e-mail: buravkova@impb.ru
Bone marrow stroma contains mesenchymal stem cells (MSC) which are precursor for at least mesenchyma-derived
cells. Recent investigations revealed a lot of questions concerning MSC biology that should be further refined. The
aim of this study was the comparative analysis of rat bone marrow stroma cells cultures. Mesenchymal precursor cells
isolated from rat bone marrow were passed up to 50 times. Comparative morphological and immunophenotypical analysis
of these cultures was carried out as well as their ability to osteogenic differentiation was studied. The isolated
cultures contained morphologically different types of cells and thus showed a high heterogenity level. Morphology of
these cell types was described. The heterogeneity level was reported to decrease over time. It was found out that
subcultures isolated from different rats shared the same immunophenotype characteristics (CD90+,
CD44+, CD54+, CD106+, CD45–, CD11b–), but differed in their
morphology as well as in ability to osteogenic differentiation. Thus MSC identification requires more specific
marker and functional tests to be used.
Key words: mesenchymal precursor cells, bone marrow, heterogeneity, mesenchymal stem cells
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