HEAT SHOCK PROTEIN OF HSP70 FAMILY REVEALED IN SOME CONTEMPORARY FRESHWATER AMOEBIDS AND IN ACANTHAMOEBA
SP. EXCYSTED FROM CYSTS ISOLATED FROM PERMAFROST SAMPLES
Yu. I. Podlipaeva,1 L. A. Shmakova,2 D. A. Gilichinski,2
A. V. Goodkov 1,*
1 Institute of Cytology RAS, St. Petersburg, and 2 Institute of Physicochemical and
Biological Problems in Soil Science RAS, Pushchino, Moscow Region;
* e-mail: pelgood@rambler.ru
A heat shock protein of HSP70 family was revealed for the first time in trophozoites of Acanthamoeba sp.
(strain Am8) excysted from cysts previously isolated from samples of permafrost aging 30 000-35 000 years. The
constitutive level of this HSP, shown by immunnoblotting in unstressed trophozoites of the ancient acanthamoebae,
much surpassed that in unstressed cells of the three examined species of contemporary freshwater amebae of the genus
Amoeba.
Key words: heat shock proteins, HSP70, Acanthamoeba, viable amoebae from permafrost,
Amoeba
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