THE NUCLEOLUS IN OOCYTES OF MULTYLAYER MOUSE FOLLICLES: TOPOGRAPHY OF FIBRILLARIN, RNA POLYMERASE I AND COILIN
G. N. Pochukalina, V. N. Parfenov
Institute of Cytology RAS, St. Petersburg;
e-mail: galina@mail.cytspb.rssi.ru
By electron microscopy, conventional fluorescence and confocal microscopy, some features of structural and
molecular organization of the nucleolus in oocyte nucleus from mouse multilayer follicles were examined. The examined
nucleolus lacks almost all characteristic nucleolar components, such as fibrillar centers, dense fibrillar and
granular components. This nucleolus consists exclusively of a homogenous filamentous material and is penetrated by
numerous interstices. Besides, a striking association of the nucleolus with coilin, a marker of Cajal bodies, was
observed. We could map the coilin accumulation in three different areas: around, in the periphery, or inside the
nucleolus. Additionally, we examined a topological relationship between coilin and two key proteins of nucleolar
transcription-processing machinery, RNA polymerase I and fibrillarin. RNA polymerase I rather than fibrillarin was
found to be colocalized with coilin. Finally, we propose that data on dynamics of coilin relation with the nucleolus
may elucidate a possible role of coilin in functional relationship between the nucleolus and Cajal bodies.
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