VARIABILITY OF CONTINUOUS INSECT CELL LINES AND THEIR IDENTIFICATION
D. Y. Panteleev,1 V. T. Kakpakov, T. V. Gorelova, B. V. Andrianov
N. I. Vavilov Institute of General Genetics RAS, Moscow;
1 e-mail: mycobiota@yandex.ru
Continuous insect cell lines make a special object of research in biology. Insect cells in the established lines
differ in the number of attributes from both normal differentiated, and embryonic cells. The period of genome
destabilization necessarily precedes cell line immortalization. Genome destabilization is manifested by changes in
genome size, cell karyotype, amplification of some retrotransposone families, and induction of their expression. The
existence of significant genetic variability in one line puts a problem of searching for invariant attributes
providing culture identification and defining the limits of normal polymorphism of cells in the culture. Using the
vast collection of insect continuous cell lines stored at the N. I. Vavilov Institute of General Genetics RAS, nine
lines were identified by RFLP method of mitochondrial DNA. Variability of DNA-polymorphisms, cellular karyology,
morphology, immunological and biochemical attribute in the culture is discussed.
Key words: continuous insect cell lines, RFLP, retrotransposones, virus persistence
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