PLANT RESISTANCE INDUCTORS AND ACTIVE FORMS OF OXYGEN. III. THE INFLUENCE OF BISOL 2 AND BAITAN ON MORPHOGENESIS
AND DEFENCE RESPONSE OF WHEAT NON-MORPHOGENIC CALLUS CELLS INFECTED WITH BUNT AGENT
N. B. Troshina, L. G. Yarullina, O. B. Surina, I. V. Maksimov
Institute of Biochemistry and Genetics, Ufa Research Center RAS;
e-mail: phyto@anrb.ru
A study was made of the influence of bisol 2 and baitan compounds on morphogenesis and defence response of wheat
calluse cells infected with bunt agent in associated with oxalate oxidase activation. After introduction of bisol and
baitan into cultivation medium, dense area with meristema zones, germs of shoots and rhizoids appeared on
non-morphogenic calluses, which correlated with enzyme activation. Parenchyma-like cells, generating hydrogen
peroxide, were seen in the site of pathogen penetration under infestation, but were never revealed in control.
Generation of hydrogen peroxide in the site of infection was accompanied with an increased oxalate oxidase activity
in the cytoplasmic fraction, and with suppression of this activity in a fraction bound to the cell wall. Both
compounds induced oxalate oxidase activity under combined cultivation of wheat calluses with bunt agent.
Key words: wheat, callus, bunt patogen, bisol 2, baitan, oxalate oxidase
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