TRANSGENERATIONAL TRANSMISSION OF BOVINE SATELLITE DNA IN TRANSGENIC MICE
N. A. Slominskaya,1 * I. O. Suchkova,1 T. A. Klinskaya,1
M. A. Zabezhinsky,2 E. L. Patkin 1
1 Research Institute of Experimental Medicine RAMS and 2 N. I. Petrov Oncological
Institute RAMS, St. Petersburg;
*e-mail: slominska@mail.ru
Genetical, cytogenetical and molecular analysis was made for 5 generations of mice transgenic for bovine
satellite DNA (Sat). In all cases transgenic mice were generated by crosses of transgenic males and females with
normal (CBA × C57Bl) mice. No abnormalities in the founder development were noticed. A normal (near 50 %) ratio
of transgenic and nontransgenic offsprings was observed in blastocysts. However, profound differences occurred in the
rate of transgene bearing offsprings, depending on the sex of grandparents rather than of parents. The grandfather
Sat transmission resulted in the appearance of 0-52.4 % transgenic grandchildren, whereas the grandmother
transmission ended in the theoretically expected rate. This means that stabilization of transsatellite took place
upon the female germ line transmission (a positive grandmother effect). It is essential that in hemizygous
transsatellite mice Sat integration led to the occurrence of mammary tumors, inflammation of uterine horns, and
infringement of mother care of transgenic females. Simultaneous FISH and G-banding showed Sat to be localized in the
internal region of chromosome 12 near Pax 9 and Brms 11 genes. Commonly, these genes are implicated in
tumorigenesis as their expression decreases. Thus, a kind of silencing effect of these genes' expression may be
supposed.
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