DYNAMICS OF RAT HEPATOCYTE HISTONE GLYCOXIDATION AFTER GENERAL X-RAY IRRADIATION
N. V. Kuleva,1 M. A. Fedorova, A. A. Frolov
Chair of Biochemistry, Biological-Soil Faculty, St. Petersburg State University;
1 e-mail: kuleva@mail.ru
Dynamics of structural parameters of hepatocyte histone glucoxidative modification 3, 9 and 24 h after general
X-ray irradiation of rats at dose 5 Gy was studied. Dynamics of these parameters (content of carbonyl groups,
bityrosyl cross-linkings, pentosidines, advanced glycation end products) was compared with alterations in DNA
structure (according to agarose gel electrophoresis) and lipid peroxidation extent (by malondialdehyde content).
Oxidative stress induced by hepatocyte irradiation results in structural damage of DNA and histones accompanied by an
increase of histone bityrosyl cross-linking and carbonyl content. The content of advanced glycation end products in
histones corresponds to the extent to DNA damage and malondialdehyde content. The described postradiation
modifications of histones may be important for regulation of chromatin function.
Key words: advanced glycation end products, DNA, hepatocytes, histones, glycoxidative modification,
X-ray irradiation
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