ULTRASTRUCTURAL CHANGES OF ENDOPLASMIC RETICULUM, GOLGI APPARATUS MEMBRANES AND MITOCHONDRIA IN THE IDENTIFIED
NEURON OF MOLLUSC AFTER CRYOPRESERVATION
E. V. Dmitrieva,1 D. A. Moshkov,2 E. N. Gakhova 1
1 Institute of Cell Biophysics RAS and 2 Institute of Theoretical and Experimental
Biophysics RAS, Pushchino, Moscow region;
1 e-mail: edmitrieva3@yandex.ru
Investigation of a possibility of long-term storage of frozen (-196 °C) viable neurons and nervous tissue is one
of the central present day problems. In this study ultrastructural changes in neurons of frozen-thawed snail brain
were examined as a function of time. We studied the influence of cryopreservation, cryoprotectant (Me2SO),
cooling to 4-6 °C, and a prolonged incubation in physiological solution at 4-6 °C on dictyosomes of Golgi apparatus,
endoplasmic reticulum (ER) cisternae and mitochondria. It has been found that responses of these intracellular
structures of cryopreserved neurons to the above influences are similar: dissociation of Golgi dictyosomes, swelling
of endoplasmic reticulum cisternae and mitochondrial cristae. Both freezing-thawing and cryoprotectant were seen to
cause an increase in the number of lysosomes, liposomes, myelin-like structures, and to form large vacuoles. The
structural changes in molluscan neurons caused by cryopreservation with Me2SO (2 M) were reversible.
Key words: cryopreservation, mitochondria, neuron, Golgi apparatus, endoplasmic reticulum
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