PARTICIPATION OF BONE-MARROW STEM CELLS IN THE DIFFERENTIATION OF mdx MICE STRIATED MUSCLE
V. M. Mikhailov, E. V. Eftifeeva, V. B. Serikov, A. E. Pereverzev, A. V. Karmanova, V. V. Zenin
Institute of Cytology RAS, St. Petersburg;
e-mail: vmikhailov@mail.cytspb.rssi.ru
Two sets of experiments were carried out. The first one involved chimeric mice, obtained by intravenously
injections of bone marrow derived cells taken from transgenic C57BL/6 mice, expressing GFP, to 5 Gy X-ray irradiated
mdx or C57BL/6 mice. In 2 months M. quadriceps femoris of chimeric mice were destroyed by surgical clamp.
Following the next 4-5 weeks, the same muscles were studied for the presence of GFP-positive striated muscle fibres.
In the case of chimeric C57BL/6 mice GFP-positive striated muscle fibres were observed in 0.3 ± 0.5 and in
0.2 ± 0.3 % of destroyed muscle, and in lateral (control) muscle, consequently. In the case of chimeric mdx mice,
positive results were observed in 1.7 ± 0.4 and in 0.5 ± 0.3 % of destroyed and control muscles, respectively. In the
second set of experiments, the GFP-positive bone marrow cells were used for multiple intramuscular injections to
M. quadriceps femoris of C57BL/6 or mdx mice in a dose of 2_105-5_105 cells per mouse. Before injection,
GFP-positive bone marrow cells were fractionated in a 63 % Percoll solution and then were exhausted from
differentiated cells by magnetic manner using CD4, CD8, CD38, CD45R, CD119, Ly-6G, and F4/80 antibodies. After 2-3
weeks, as many as 0.15 ± 0.40 and 0.1 ± 0.2 % of GFP-positive muscle fibres were found in injected and control
muscles of C57BL/6 mice, respectively. In the case of mdx mice, the frequency of GFP-positive striated muscle fibres
was 2.0 ± 0.8 and 1.2 ± 0.6 % for injected and control muscles, respectively. A conclusion is made that bone marrow
stem cells can take part in differentiation of mdx mouse muscles after their delivery by needle injections.
Key words: chimeric GFP-positive C57BL/6 and mdx mice, stem cells, skeletal muscle, regeneration,
differentiation
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