VARIATION OF MICROSATELLITES BM224 AND BCAL7 IN POPULATIONS OF GREEN TOADS (BUFO VIRIDIS
COMPLEX) WITH VARIOUS NUCLEAR DNA CONTENT AND PLOIDY
S. N. Litvinchuk,1 Ju. M. Rosanov,1 N. M. Usmanova,1
L. Ja. Borkin,2 L. F. Mazanaeva,3 V. I. Kazakov 1
1 Institute of Cytology RAS, 2 Zoological Institute RAS, St. Petersburg, and
3 Dagestan State University, Makhachkala;
e-mail: slitvinchuk@yahoo.com
We studied variation of microsatellites BM224 and Bcal7 in three species of the Bufo
viridis diploid-polyploid complex. We found that locus Bcal7 in all examined samples was monomorphic.
Three alleles of microsatellite BM224 were found. Among tetraploid toads, the western species B.
oblongus had only one allele variant, whereas the eastern species B. pewzowi had two other alleles.
Similar distribution of alleles was observed in triploid specimens, collected in the area borders of tetraploid and
diploid species. Among samples of diploid toad B. viridis, we found all three allele variants of
microsatellite BM224. Their distribution was geographically determined. A comparison of allele distribution
with genome size variation in diploid toads showed very similar patterns.
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