HUMAN LARGE INTESTINE ADENOCARCINOMA CELLS (CaCo-2) IN THE PROCESS OF CULTIVATION
E. M. Karalova,1 L. O. Abroyan,1 L. A. Hakobyan,1
M. H. Gasparyan,1 Z. A. Karalyan,1 N. G. Jaghatspanyan,2
Z. R. Ter-Pogossyan,2 L. A. Kamalyan,2 Yu. A. Magakyan 1
1 Institute of Molecular Biology NAS of Armenian Republic and
2 the Fanarjan Scientific Center of Oncology, Ministry of Health of Armenian Republic, Yerevan
Using cytomorphometry and cytophotometry cells of human large intestine adenocarcinoma (CaCo-2) were studied
under condition of a 10 day cultivation. A reverse dependence was established between proliferative activity and
monolayer density. The increase of the latter inhibits proliferation and promotes the formation of islets of
polymorph cells. 2c-cells could be seen only at the beginning of culture growth; a larger part of cells polyploidized
by cell blocking in G2-phase. These cells do not divide, which is testified by the absence of 2c-cells,
but some part of 4c-cells start the next cycle, accumulates 8n-DNA and then divides, replenishing the 4c-cells
population. In the process of cultivation, we observed an increase in the number and total volume of nucleoli in the
nuclei, and a rise in DNA amount in the perinucleolar chromatin. The formation of numerous 4c-cells with
multi-nucleolar nuclei may define an increase of functional activity of CaCo-2 culture as the whole, whereas the
formation of separated groups of such cells in the monolayer may denote a possible initiation of their
differentiation.
Back
Contents
Main
|