Vol. 48 (2006), N 4, p. 315-319
HUMAN LARGE INTESTINE ADENOCARCINOMA CELLS (CaCo-2) IN THE PROCESS OF CULTIVATION

E. M. Karalova,1 L. O. Abroyan,1 L. A. Hakobyan,1 M. H. Gasparyan,1 Z. A. Karalyan,1 N. G. Jaghatspanyan,2 Z. R. Ter-Pogossyan,2 L. A. Kamalyan,2 Yu. A. Magakyan 1

1 Institute of Molecular Biology NAS of Armenian Republic and 2 the Fanarjan Scientific Center of Oncology, Ministry of Health of Armenian Republic, Yerevan

Using cytomorphometry and cytophotometry cells of human large intestine adenocarcinoma (CaCo-2) were studied under condition of a 10 day cultivation. A reverse dependence was established between proliferative activity and monolayer density. The increase of the latter inhibits proliferation and promotes the formation of islets of polymorph cells. 2c-cells could be seen only at the beginning of culture growth; a larger part of cells polyploidized by cell blocking in G2-phase. These cells do not divide, which is testified by the absence of 2c-cells, but some part of 4c-cells start the next cycle, accumulates 8n-DNA and then divides, replenishing the 4c-cells population. In the process of cultivation, we observed an increase in the number and total volume of nucleoli in the nuclei, and a rise in DNA amount in the perinucleolar chromatin. The formation of numerous 4c-cells with multi-nucleolar nuclei may define an increase of functional activity of CaCo-2 culture as the whole, whereas the formation of separated groups of such cells in the monolayer may denote a possible initiation of their differentiation.


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