PECULIARITIES OF REPETITIVE DNA LOCALIZATION IN PERICENTROMERIC HETEROCHROMATIN OF MALARIAL MOSQUITOES OF THE
"ANOPHELES MACULIPENNIS" COMPLEX
O. G. Grushko,1 A. M. Rusakova,1, 2, * M. V. Sharakhova,1
I. V. Sharakhov,1, 2 V. N. Stegniy 1, 2
1 Research Institute of Biology and Biophysics, Tomsk State University, and
2 Chair of Cytology and Genetics, Tomsk State University;
* e-mail: gene@res.tsu.ru
Distribution of eight fragments of conserved repetitive DNA from pericentromeric heterochromatin of chromosome
2 of Anopheles atroparvus has been investigated by in situ hybridization on polytene chromosomes of An.
atroparvus and An. messeae. We have shown that heterochromatic regions of all chromosomes both in An.
atroparvus and An. messeae vary in combinations of, at least, conserved repeats. Some repeats have been
found only in pericentromeric heterochromatic regions of chromosomes 2 (clones Atr2R-46a, Atr2R-73, Atr2R-85a in
An. atroparvus and Atr2R-25 in An. messeae). Others have been found in two (clones Atr2R-25a and
Atr2R-90 in An. atroparvus, Atr2R-25a in An. messeae) and more (clones Atr2R-118, Atr2R-136 in An.
atroparvus, Atr2R-73 in An. messeae) pericentromeric heterochromatic regions of chromosomes. DNA
comparison of pericentromeric heterochromatic regions of chromosomes in species of the "Anopheles
maculipennis" complex is species- and chromosome-specific, due, in particular, to different maintenance of
conserved repeates.
Key words: polytene chromosomes, pericentromeric heterochromatin, conserved repetitive DNA,
"Anopheles maculipennis" complex
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