Vol. 48 (2006), N 2, p. 161-168

ELABORATION OF BIODEGRADABLE POLYMER SUBSTRATE FOR CULTIVATION OF HUMAN DERMAL FIBROBLASTS Yu. A. Shved,1,* L. V. Kukhareva,2 I. M. Zorin,1 A. Yu. Solovjov,3 M. I. Blinova,2 A. Yu. Bilibin,1 G. P. Pinaev 2 1 Chemical Faculty, St. Petersburg State University, 2 Institute of Cytology RAS, and 3 Institute of High Molecular Compounds RAS, St. Petersburg; e-mail: ulychka@rambler.ru The influence of polylactic acid (PLA) surface films on the pattern of cell behavior was studied. The human dermal fibroblasts were cultivated on PLA covered glasses. The hydrophobic nature of PLA films depends on the availability of polymer solvent in the film preparation. PLA films obtained from a more polar solvent-aceton-appeared to be more hydrophilic than those obtained from methylene chloride. More hydrophilic polymer films also appeared to be more preferable for cell cultivation, and human dermal fibroblasts demonstrated a better adhesion and proliferation on hydrophilic rather than on hydrophobic PLA films. Key words:  polylactic acid, acetone, methylene chloride, fibroblasts Back    Contents    Main