GENERATION OF REACTIVE OXYGEN SPECIES IN MONOCYTES AT ADHESION TO GLASS
A. A. Kryukov, G. N. Semenkova, S. N. Cherenkevich
Department of Biophysics, Belorussian State University, Minsk;
e-mail: Krjukovaa@mail.ru
Processes of oxygen activation in monocytes stimulated with adhesion to glass were studied by methods of
luminol-dependent and lucigen-independent chemiluminescence. It was shown that monocyte chemiluminescence was
caused by cell adhesion to glass surface. Generation of reactive oxygen species at monocyte adhesion to glass was
dependent on calcium ion concentration in the medium. The increase in the level of cytosolic calcium, as the
extracellular calcium concentration elevated, was accompanied by the activation of phospholipase A2,
5-lypoxygenase and cycloxygenases. Magnesium ions exerted no influence on oxygen activation by cells. Incubation
of cells in glucose-free medium, or the addition of glycolysis blocker (2-deoxy-D-glucose) to cell suspension
led to a decrease in chemiluminescence intensity. By means of inhibitory analysis, it has been established that
processes of oxygen activation are related to arachidonic acid metabolism, and depend on the activity of
phospholipase A2.
Key words: monocytes, NADPH oxidase, reactive oxygen species, adhesion, chemiluminescence
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