MICROMORPHOLOGICAL FEATURES OF PLEUROTUS PULMONARIUS (FR.) QUEL. AND P. OSTREATUS (JACQ.)
P. KUMM. STRAINS IN PURE AND BINARY CULTURE WITH YEASTS
O. V. Kamzolkina,1 A. N. Grishanina,2 E. V. Pancheva,1
V. N. Volkova,1 M. V. Kozlova 1
1 M. V. Lomonosov Moscow State University, and 2 N. N. Blokhin Russian Oncological
Centre, Scientific Research Institute, Moscow;
e-mail: o-kamzolkina@yandex.ru
Micromorphology of oyster mushrooms Pleurotus ostreatus (Jacq.) Quel. and P. pulmonarius (Fr.) Quel.
was studied in pure and binary culture with yeasts (Cryptococcus laurentii 1629, Rhodotorula minuta 2790,
Sporidiobolus salmonicolor Ö 31A-11, Nandida krusie 3452, Pichia holstii 3438). The cultures were cultivated
on malt-agar and water agar. Various mycelial structures were described: strands, rings, thin searching mycelium,
clamps, crystals, head-like offshoots, mycelial fragments, chlamydospores, and coralloid hyphae. Vegetative
mycelia interact in different ways (forming anastomoses, strands, system of thin anucleate hyphae) within the
same culture. Head-like offshoots of mycelial cells, previously regarded as spores of asexual reproduction, appeared
to lack nuclei and to be filled with polyphosphates. Coralloid hyphae, which induce yeast cell lysis after
direct contact, were detected only in binary culture with yeasts under condition of nitrogen deficit. The same
way of feeding is typical for carnivorous mushrooms.
Key words: micromorphology, Pleurotus pulmonarius, Pleurotus ostreatus, binary culture,
carnivorous ability of Pleurotus
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