Vol. 48 (2006), N 11, p. 958-966

RETARDED EXCISION OF PYRIMIDINE DIMERS IN HUMAN UNSTIMULATED LYMPHOCYTES Sergei A. Snopov,1,* Len Roza,2 Frank R. de Gruijl 3 1 Institute of Cytology RAS, St. Petersburg, Russia, 2 Department of Nutritional Physiology, TNO Nutrition and Food Research, Zeist, The Netherlands, and 3 Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands; * e-mail: snopov@mail.cytspb.rssi.ru Using immuno-labelling of cyclobutane pyrimidine dimers (CPDs) in nuclei of peripheral lymphocytes after their UVC-irradiation and cultivation, we have found that within the first four hours of cultivation the CPD-specific fluorescent signal from cell nuclei increased. Earlier, a similar increase in binding of antibody specific for pyrimidine (6-4) pyrimidone photoproducts to undenatured DNA isolated from UV-irradiated Chinese hamster ovary cells was reported (Mitchell et al., 1986). Our experiments showed that nucleotide excision repair enzyme might induce such of DNA modification in lymphocyte nuclei that increased specific antibody binding to DNA fragments with lesions. We suggest that enzymatic formation of open structures in DNA predominated qualitatively over dual-incision and excision of these fragments, and resulted in the enhanced exposure of the pyrimidine dimers in nuclei to specific antibodies. The results evidence that nucleotid excision repair in unstimualted human lymphocytes being deficient in dual incision and removal of UV-induced DNA lesions appear to be capable of performing chromatin relaxation and pre-incision uncoiling of DNA fragments with lesions. Back    Contents    Main