REGULATION OF MORPHOLOGICALAND FUNCTIONAL PROPERTIES OF ASTROGLIAL CELLS BY HYDROGEN PEROXIDE
T. A. Kulagova,1 G. N. Semenkova,1 Z. B. Kvacheva,2
S. N. Cherenkevich 2
1 Department of Biophysics, Physical Faculty, Belarusian State University,
Minsk, Belarus; and 2 Research Institute of Epidemiology and Microbiology, Republic of Belarus
Ministry of Health, Minsk, Belarus;
e-mail: tatyana_kulagova@tut.by
Effects of hydrogen peroxide on morphological characteristics, proliferation index, menadione-dependent
lucigenin-enhanced chemiluminescence of C6 glioma cells were studied. It was established that
H2O2 at 5 · 10-7-1 · 10-8 M concentrations acts as a regulator
of morphological and functional properties of astrocytes by inducing their reactivation that is manifested as a cell
body hypertrophy and an increase of proliferative activity and of menadione-dependent production of superoxide
(O2•-). Cytodestructive action of hydrogen peroxide at a concentration higher than 1 mM
on C6 glioma cells shows itself as a decrease of their proliferation index and the ability to generate
O2•- under menadione action. Using lipopolysaccharide B as a functional stimulator it
has been shown that H2O2 modifies signaling pathways leading to the increase of mitotic
activity of C6 glioma cells and decreases the yield of lucigenin-enhanced chemiluminescence of astrocytes under
menadione action to the level of control values.
Key words: C6 glioma cells, hydrogen peroxide, lipopolysaccharide, proliferative index,
chemiluminescence
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