DYNAMIC OF DNA-BINDING ACTIVITY OF TRANSCRIPTION FACTORS IN A431 CELLS DURING THEIR SPREADING ON IMMOBILIZED
LIGANDS
A. N. Emelyanov, O. A. Petukhova,1 L. V. Turoverova, I. V. Kropacheva, G. P. Pinaev
Institute of Cytology RAS, St. Petersburg;
1 e-mail: olvial@mail.cytspb.rssi.ru
Dynamics of actin cytoskeleton in A431 cells and specific NF-ęB SRF and AP-1 DNA-binding activities were studied
during a 2 h spreading of these cells on fibronectin, laminin-2/4 or an antibody to epidermal growth factor receptor.
Cell spreading was shown to be accompanied by sequential formation of actin cytoskeleton structures, whose spatial
organization depends on the type of immobilized ligand. We have determined the time intervals, within which certain
forms of cytoskeleton do not change qualitatively and are specific for dominant part of cell population. It has been
shown that DNA-binding activities of the above transcription factors studied oscillate during cell spreading. The
cycles of DNA-binding activity were found to be equel to 15-40 min. The character of oscillations depends on both
transcription factor and ligand type. The temporal comparison of presses of actin cytoskeleton formation and
DNA-binding activity of NF-ęB and SRF times suggest that actin cytoskeleton reorganization may be presumably
associated with activation of NF-ęB and SRF.
Key words: transcription factors, adhesion, extracellular matrix, actin dynamics
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