PARTICIPATION OF TRANSFUSED BONE MARROW CELLS IN REPARATIVE OSTEOHISTOGENESIS
R. V. Deev,1 * N. V. Tsupkina,2 V. N. Serikov,3
V. G. Gololobov,1 G. P. Pinaev 2
1 Russian Military Medical Academy, 2 Institute of Cytology RAS, and
3 Medical Academy of Post-Graduatc Education, St. Petersburg, Russia;
* e-mail: romdey@inbox.ru ;
www.osteon.spb.ru
The participation of skeletal tissue cell precursors in the repairing regeneration of bone tissue was studied.
Bone marrow was taken from donor animals - mice of C57Bl/6-TgN(ACTbGFP)lOsb line (The Jackson Laboratory Bar Harbor ME USA line).
Nucleated cell fraction was isolated by centrifugation on a density percoll gradient. Recipient mice C57B1/6 line were irradiated
by 7.0-7.5 Gr dose. Intravenous infusion of donor cells and osteoclasts of tibia was done after irradiation of recipient mice.
Histological preparations of bone regenerate tissues were studied on 15, 30, and 60 days by confocal microscopy. Donor cells were
found as skeletal tissue precursors into periost, endost, bone marrow, and as differentiated cells of newborn tissue of regenerate
- osteoblasts, osteocytes, chondrocytes. The data obtained indicate that part of donor bone marrow cells are able to progressive
differentiation under recipient bone fractures.
Key words: stromal stem cells, mesenchymal stem cells, regeneration of bone tissue, osteogenesis, GFP, cell
transplantation
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