DIFFUSIONAL ATTENUATION OF PROTON ECHO AND CONTROL OF CHANGES IN THE CYTOSKELETON
HYDRATION LEVEL
G. A. Velikanov,1 L. P. Belova, E. M. Gaponenko
Institute of Biochemistry and Biophysics, Kazan' Scientific Center, RAS, Kazan', Russia;
1 e-mail: velikanov@mail.knc.ru
Using the pulsed NMR-method with pulse gradient of the magnetic field, a study was made of changes in
diffusional attenuation (DA) of proton echo in germs of triticum grains under the influence of agents depolymerizing
cytoskeleton elements or inhibiting their ATP-activity. At a short diffusion time, we observed respective alterations of
NMR-population in a fraction of cell water molecules contributing to DA at large values of pulse gradient. It is shown that
the presence in DA of a site with essentially smaller coefficient of self-diffusion, as compared with basic cell water
fraction, is not a consequence of restricted diffusion phenomenon. The observed changes in NMR-population of the appropriate
fraction of cell water molecules are interpreted as changes in the level of cytoskeleton hydratation occurring under the
influence of the used agents. A hypothesis is proposed for the functional role of similar changes in the level of hydratation
of cytoskeleton elements located inside plamo-desmata in the mechanism of plasmodesmatal water permeability regulation.
Key words: cytoskeleton, hydratation inhibitors, NMR-method
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