PECULIARITIES OF THEOPHYLLINE AND PROLACTIN INTERACTION AND CALCIUM FLUCTUATION FROM INTRACELLULAR STORES OF
PORCINE OOCYTES IN THE PRESENCE OF ESTRADIOL
V. Yu. Denisenko,1 T. I. Kuz'mina
All-Russian Research Institute of Farm Animal Genetics and Breeding, St. Petersburg-Pushkin,
Russia;
1 e-mail: vitald@fromru.com
Using a fluorescent dye chlortetracycline, a study was made of the effect of estradiol on the interaction of
theophylline and prolactin in the course of Ca2+ exit from intracellular stores of pig oocytes, isolated from ovaries
at the stage of follicle growth. It is shown that in the presence of estradiol, prolactin does not stimulate Ca2+
exit from intracellular stores of pig oocytes. The action of theophylline similarly does not stimulate Ca2+ exit. Unlike, a
joint effect of theophylline and prolactin on pig oocytes in the presence estradiol stimulated Ca2+ exit from
intracellular stores of pig oocytes. These data demonstrated the influence of estradiol on theophylline and prolactin
stimulated Ca2+ exit from intracellular stores of pig oocytes.
Key words: estradiol, theophylline, prolactin, calcium, pig oocytes
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