DEPENDENCE OF Ca2+ RELEASE FROM INTRACELLULAR STORES ON NADH AND FAD LEVELS IN FERTILIZED AND
UNFERTILIZED BOVINE OOCYTES
V. Yu. Denisenko,1 T. I. Kuz'mina, O. V. Shokin
All-Russian Research Institute of Farm Animal Genetics and Breeding, St. Petersburg-Pushkin,
Russia;
1 e-mail: vitald@fromru.com
Relation between NADH and FAD concentrations and the quantity of calcium released from intracellular stores in
fertilized and unfertilized bovine oocytes was investigated using luminescent analysis. Inhibition of Ca2+ exit from
intracellular stores was detected in degenerative oocytes at metaphase II and 2-cell embryos. The intensity of both NADH and FAD
fluorescence increased in 2-cell degenerated embryos, whereas the increase in only NADH fluorescence intensity occurred in
degenerated oocytes at metaphase II stage. Degeneration exerted no influence on NADH fluorescence intensity or Ca2+
exit from intracellular stores, whereas a decreased FAD fluorescence intensity was noted in degenerated pronuclei. The obtained
data testify that in degenerated zygotes and early embryos Ca2+ release may occur from different intracellular stores.
Key words: NADH, FAD, calcium, oocytes, embryos of bovine
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