ANALYSIS OF TUMOR CELL PROLIFERATIVE ACTIVITY IN AGGRESSIVE HUMAN LYMPHOMAS: THE INFLUENCE OF REACTIVE CELL
ADMIXTURE ON THE ASSESSMENT OF PROLIFERATIVE INDICES
E. V. Sheval, 1, 2 J. V. Churakova,3
I. A. Vorobjev 1, 3
1 A. N. Belozersky Institute of Physico-Chcmical Biology, Moscow State University,
2 Institute of Agricultural Biotechnology, RAAS, and 3 Hematology Research Center, RAMS, Moscow, Russia;
e-mail: esheval@mail.ru
Uncontrolled proliferation is one of the main features of tumor cells, and therefore proliferative indices
provide prognostic information, which might be valuable for treatment selection. However, in aggressive human lymphoid tumors,
an admixture of normal (reactive) cells may be available in all organs infiltrated by neoplastic cells. We have presented data on
determining proliferative activity using Ki-67 immunostaining, and demonstrated that an admixture of reactive cells could exert
significant influence on the assessments of proliferative indices. We developed an experimental approach, which makes it possible
to select neoplastic cells from the overall lymphoid population and to assess their proliferative activity - tumor cell
proliferative indices. Tumor cell profliferative indices determined in large cell lymphomas might be significantly higher than
proliferative indices of overall populations, and the use of tumor cell proliferative indices will result in a more accurate
assessment of disease prognosis.
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