THE GENETIC NATURE OF ABNORMAL LARVAL DEVELOPMENT IN THE PROGENY OF
l(1)ts403(sbr10) FEMALES OF DROSOPHILA MELANOGASTER
O. M. Pougatcheva, L. A. Mamon
St. Petersburg State University, St. Petersburg, Russia;
e-mail: olga@OP10659.spb.edu,
mamon@LM2010.spb.edu
In Drosophila melanogaster the small bristles (sbr) gene is vital and evolutionary conservative
and controls nuclear export of mRNA. Sbr mutant alleles had a broad pleiotropic effect. High frequency of abnormal larva dying
(up to 18 %) at the first instar stage in progeny of heat shock (37 °C, 1 h) treated mutant females is one of the most
interesting l(1)ts403(sbr10) allele effects. Abnormal larvae display characteristic phenotype that involves
the Malpighian tubules defect. Using interphase FISH method (fluorescence in situ hybridization), we showed that abnormal
larvae had monosomy on chromosomes 2 and 3. DNA content in neuroblast interphase nuclei of abnormal larvae is 2.1 times less
than in normal larvae. We suggest that abnormal larvae could be full or mosaic haploids that appeared as a result of maternal
genome loss during fertilization or the mitotic division. Larvae with the same abnormalities appear in a progeny of females with
different genotypes mating with males carrying compound chromosomes 2 or 3. FISH analysis showed that such larvae had monosomy
only on a chromosome that is compound in paternal strain. Thus, monosomy on large autosomes may cause aspecial phenotype of
abnormal larvae in D. melanogaster.
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