PECULIARITIES OF CHANGING EXTRACELLULAR MATRIX OF CELLS DURING DEVELOPMENT OF SUSPENSION CULTURE OF
TRITICUM TIMOPHEEVII ZHUK.
I. A. Larskaya,1 T. S. Barisheva, A. I. Zabotin
Kazan' Institute of Biochemistry and Biophysics, RAS, Russia;
1 e-mail: larskaja@mail.knc.ru
A study was made of the contents of the main polysaccharide fractions in the cell wall, and extracellular
polysaccharides, and of the activity of cell wall enzymes during cultivation of suspension culture of cells of the winter wheat
Triticum timopheevii Zhuk. It was shown that within 3 days of cultivation (a phase enriched in dividing cells), on the
background of increased callose contents in plant cells, amounts of pectins and hemicelluloses extracted by 4N alkali decreased.
The content of polysaccharides reached its initial level by the end of cultu-ring. A parallel analysis of glycosidase activity
in cell walls has shown their considerable activation at the stage enriched by dividing cells, which decreased at a transition
of culture into the stationary level. The increased activity of hydrolyzing enzymes was combined with an increased efflux of
extracellular polysaccharides into culture medium. The detected changes in polysaccharide composition of the cell wall at the
first phase indicate its qualitative changes during cell wall reconstruction at the beginning of cytokines, whereas extensive
expansion of cell wall was seen on the phase of elongation.
Key words: cell wall, polysaccharides, suspension culture, enzymes of cell wall, extracellular polysaccharides
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