SCORING CRITERIA OF ANEUPLOIDY FREQUENCY IN INTERPHASE NUCLEI BY FISH-ANALYSIS
V. A. Timoshevsky, S. A. Nazarenko
Research Institute of Medical Genetics, Tomsk Research Center, AMS, Russia;
e-mail: snaz@img.tsu.ru
Principles and approaches of assessment of numerical chromosome abnormalities in interphase nuclei by
fluorescent in situ hybridization technology are reviewed. The authors' own scoring criteria of results of hybridization of
centromere-specific DNA-probes with chromosomal targets in somatic cells by dual color FISH-analysis are suggested. Use of
these scoring criteria allows to reduce the level of artificial hypoploidy, whose its frequency becomes similar to that of
hyperploidy. This fact indicates that chromosomal nondisjunction is a major mechanism of aneuploidy induction, rather than of
chromosome loss.
Key words: aneuploidy, interphase cytoogenetic analysis, fluorescence in situ hybridization
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