TEMPERATURE IMPACT ON LOCALIZATION OF "FREE" PHENOLIC COMPOUNDS IN THE ROOT TISSUES AND DEFORMATION OF
ROOT HAIRS IN PEA SEEDLINGS INOCULATED BY RHIZOBIUM
L. E. Makarova, V. N. Nurminsky
Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of RAS, Irkutsk, Russia;
e-mail: ustaft@sifibr.irk.ru
The study was focused on localization of "free" phenolic compounds in pea Pisum sativum L. seedling
roots grown at 22 and 8 °C 24 h after their inoculation with Rhizobium leguminosarum bv. viceae bacteria. A
comparison of phenolic compound distribution along the root in root tissues, and results of observation of root hair development
on the root surface, response of root hairs to inoculation, manifesting itself in various deformation degree (bends, twists, ect.)
enabled us to reveal differences between roots grown at different temperatures. These differences are basically referred to a
sector localized 0-5 mm away from the root tip containing meristematic and extending cells. A distribution of phenolic compounds
in sectors with root hairs responding to inoculation by various degrees of contortion practically did not depend on the
temperature of plant growth. The evidence provided in the course of this work enabled us to suggest that inhibition of pea root
infection at low temperature is caused by decelerated growth processes characteristic of both the root itself and root hairs,
as well by a slow increase in the root hair zone.
Key words: Pisum sativum L., phenolic compounds localization,
Rhizobium leguminosarum bv. viceae, inoculation, root hair, temperature
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