THE INFLUENCE OF MESENCHYMAL STEM CELLS ON BONE TISSUE REGENERATION UPON IMPLANTATION OF DEMINERALIZED
BONE MATRIX
P. V. Kruglyakov, I. B. Sokolova, N. N. Zin'kova, S. V. Viide, N. N. Cherednichenko,
T. V. Kislyakova, D. G. Polyntsev
Trans-Technologies, Ltd., St. Petersburg, Russia;
e-mail: pitkus@alkorbio.ru
Mesenchymal stem cells (MSC) are resident pluripotent cells of bone marrow stroma. MSC are able to
differentiate into chondroblasts, adipocytes, neurons, glia, cardiomyocytes, or osteoblasts. The problem of MSC usage in cell
therapy of bone defects is widely discussed at present. The experiments were carried out using rats of inbred line Wistar-Kyoto.
MSC were isolated from bone marrow and cultivated in vitro. Demineralized bone matrices (DBM) were obtained from
parietal bones of rats and hens. Part of DBM was loaded with MSC. Bone defects were made in cranium parietal regions.
DBM with or without MSC or metal plates were transplanted in these regions. It was shown that the application of MSC increased
angiogenesis and osteogenesis in the damaged bone. The implantation of rat's DBM with MSC led to the formation of a full
value bone. MSC suppressed inflammation, when transplantation of hen's DBM was carried out. The application of MSC always
improved bone tissue regeneration.
Key words: mesenchymal stem cells, demineralized bone matrix, bone defect, transplantation
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