PECULIARITIES OF NITROBLUE TETRAZOLIUM REDUCTION BY HUMAN BLOOD NEUTROPHILS. II. THE INFLUENCE OF SODIUM AND
POTASSIUM IONS
I. G. Gerasimov, D. Yu, Ignatov
Research Institute of Medical Family Problems, Donetsk State Medical University, Ukraine;
e-mail: DmiL@rambler.ru
Activity of blood neutrophils of 18 healthy volunteers (4 men and 14 women aged from 20 to 32 years) was
investigated in the Nitroblue Tetrazolium (NBT) reduction reaction in Na- and K-phosphate buffers (0.1 M, pH 7.3) after a
preliminary incubation of heparinized blood (37 °C, 0 - 7 h). It was found that the percentage of NBT-positive neutrophils in
the presence of Na+ is higher than in the presence of K+. The dependence of percentage of NBT-positive
neutrophils on the reaction time is described by a kinetic equation with constants k1 and k2,
characterizing accordingly, speeds of NBT absorption and reduction by neutrophils. It has been shown that k1,
k2 and k1/k2 values differ in potential phagocytes and in neutrophils,
which are not capable of adherence. The kinetics constants of the latter cells do not depend on both cations and incubation
time, while the percentage of NBT-positive potential phagocytes, and their k1 and k2 in the
presence of Na+ are higher than in the presence of K+, and the values of described parameters are changed
during incubation. During incubation the percentage of NBT-positive neutrophils increases to result in a partial aggregation of
thrombocytes, as a consequence of excretion in the blood of products involved in this process.
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