CHANGES IN CYTOKINE CONTENT IN THE PERIPHERAL BLOOD OF VOLUNTEERS AFTER THEIR EXPOSURE TO POLYCHROMATIC
VISIBLE AND INFRARED LIGHT
N. A. Zhevago,1 K. A. Samoilova,1,* K. D. Obolenskaya,1
D. I. Sokolov 2
1 Institute of Cytology, RAS, and 2 Research Institute of Obstetrics and
Gynccology after D. O. Ott, St. Petersburg, Russia;
* e-mail: kirasam@mail.cytspb.rssi.ru ,
samoilova3@yandex.ru
Anti-inflammatory, immunomodulating and wound-healing effects of visible and infrared (IR) radiation from laser
and non-laser sources are widely used in current medicine. However, the role of pro- and anti-inflammatory cytokines in
development of these effects has been poorly studied. A randomized, placebo-controlled, double blind study was made. Using ELISA,
the content of 10 cytokines was studied in the peripheral blood of volunteers after a single and four daily irradiations of the
sacral area (D = 15 cm) with polychromatic visible + IR polarized light (480-3400 nm, 12 J/cm2). The phototherapeutic
sessions were accompanied by four blood exfusions for the study (to a total volume of 80 ml). In the control (placebo) group,
irradiation was imitated, and blood samples of the same volume were drawn at the same time intervals as in volunteers of the main
group. A fast decrease in the level of pro-inflammatory cytokines was revealed as soon as in 0.5 h after the irradiation. This
level was retained until the end of the phototherapeutic course. At the parameters exceeding the norm, the contents of
TNF-α, IL-6 and IFN-γ fell, on average, by 34, 12 and 1.5 times, respectively. By the end of the course, the levels of
IFN-γ and of IL-12 decreased by 5 and 15 times, respectively. A fast decrease (by two-fold) was also characteristic of normal
values of IL-6. Neither IL-1β, nor IL-2 were detected in blood plasma of the examined people both before and after the
irradiation. In parallel with a decrease in the proinflammatory factor levels the amount of anti-inflammatory cytokines was
found to rise: that of IL-10 - by 2.7-3.5 times in 0.5 h and at later terms at the initially normal parameters, and that of
TGF-β1 - by 1.4-1.5 times at the initially decreased level. The IL-4 content did not change. A characteristic feature of the
light effect was a fast rise of IFN-γ amount - by 3.3-4.0 times in individuals with its initially normal level, with no
changes in IFN-α content. The above-reported regularities of the light effects were also recorded at a direct
(in vitro) irradiation of the examined volunteers' blood, as well as on addition of irradiated blood to a 10-fold volume
of non-irradiated autologous blood, i. e. at a modeling of mixing, of a small amount of transcutaneously photomodified blood
with its main circulating volume in the vascular bed of an irradiated person. Such a similarity of effects in blood following
its irradiation in vivo and in vitro enables us to associate the fast changes of the cytokine content in the
entire volume of peripheral blood with the transcutaneous photomodification of its small amounts, and with a "transfer" of the
light effects by photomodified blood to the whole pool of circulating blood.
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