THE INFLUENCE OF MESENCHYMAL STEM CELL TRANSPLANTATION TIME ON MYOCARDIAL REPARATION IN
RAT EXPERIMENTAL HEART FAILURE
P. V. Kruglyakov,1 I. B. Sokolova,1 Kh. K. Amineva,2
N. N. Nekrasova,1 S. V. Viide,1 N. V. Cherednichenko,1 A. Yu. Zaritsky3
E. N. Semernin3 T. V. Kislyakova,1 D. G. Polyntsev 1
1 Trans-Technologies Ltd., 2 Municipal Hospital N. 23, St. Petersburg, and
3 St. Petersburg State Medical University, Russia;
Mesenchymal stem cells (MSCs) have an ability to migrate in the organism to injured tissue to exert influence on inflammation and reparation in
these regions. The aim of this study was to determine the optimal time of MSCs transplantation for myocardial reparation in rat experimental heart failure.
The experiments were carried out on inbred line Wistar-Kyoto rats. Myocardial experimental infarction (El) was induced by ligation of the left descending
coronary artery. MSCs were isolated from bone marrow, cultivated in vitro and injected into the tail vein on the day of experimental infarction operation.
It was shown that the time of MSCs transplantation exerted an essential influence on angiogenesis in a damaged myocardium, on ventricular dilatation and
morphological structure of the scar. The best time for MSCs transplantation was determined within two days before El, and seven days after El.
As a result, the overload of the border zone of infarct region decreased, and no features of infarction relapse were shown in the border zone.
Key words: mesenchymal stem cells, capillaries ventricular dilatation
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