EFFECT OF ESTRADIOL ON STIMULATED THEOPHYLLINE AND PROLACTIN Ñà2+ EXIT FROM INTRACELLULAR STORES OF PIG
OOCYTES
V. Yu. Denisenko,1 T. I. Kuzmina
All-Russian Research Institute of Farm Animal Genetics and Breeding, Pushkin, St. Petersburg, Russia;
1 e-mail: vitald@fromru.com
Effect of estradiol on stimulated theophylline and prolactin Ñà2+ exit from intracellular stores of
pig oocytes was investigated using fluorescent dye chlortetracycline. It was shown that in the presence of estradiol neithert
theophylline nor prolactin stimulated Ñà2+ exit from intracellular stores of oocytes. Unlike, the common action of
theophylline and prolactin, also in the presence of estradiol, stimulated Ñà2+ exit from intracellular stores.
Inhibition of protein kinase Ñ inhibits Ñà2+ exit from intracellular stores in common action of theophylline and prolactin.
These data suggest an obvious influence of estradiol on Ñà2+ exit from intracellular stores of pig oocytes stimulated by
theophylline and prolactin.
Key words: estradiol, theophylline, prolactin, calcium, pig oocytes
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