CHARACTERISTICS OF HUMAN EMBRYONIC NEURONAL CELLS PROCURED BY NON-ENZYME METHOD
A. N. Sukach
Institute for Problems of Cryobiology and Cryomcdicinc, National Academy of Sciences of the Ukraine,
and Research Institute of Biology of Kharkiv National University after V. N. Karazin, Kharkiv, the Ukraine;
1 e-mail: an_sukach@yahoo.co.uk
Isolation and culturing of human neuronal progenitor cells is of significant value for both fundamental research and
therapeutic purposes. In this work, human embryonic neuronal cells were characterized as a heterogeneous population of progenitor cells
with various differentiation potentials. During in vitro culturing the cells are capable of re-inoculating, proliferating,
differentiating and migrating. While differentiating, these cells form neurons and glial cells. The present research demonstrates
that depending upon the culturing conditions the embryonic neuronal cells may cither form floating aggregates (incubation with
embryonic serum), attach (incubation without serum), proliferate, or form ncurospheres. Besides, peculiarities of aggregate
differentiation during their incubation under various media are described.
Key words: embryo, progenitor cell, stem cell, aggregate, neurosphcre, differentiation, cell culture
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