Vol. 47 (2005), N 3, p. 243-248

SPECIFIC DNA SEQUENCES ARE ASSOCIATED WITH NUCLEAR ENVELOPES OF PSEUDONURSE CELLS IN DROSOPHILA MELANOGASTER OTU 11 I. V. Sharakhov,1 E. M. Bariclieva,2 S. S. Bogachev,2 P. A. Fisher,3 E. R. Lapik,2 V. A. Rogachev,2 T. E. Sebeleva 2 1 Institute of Biology and Biophysics, Tomsk State University, 2 Institute of Cytology and Genetics, Siberian Branch of RAS, Novosibirsk, Russia, and 3 Department of Pharmacological Sciences, University of New York at Stony Brook, Stony Brook, USA; e-mail: owk_nsk@hotbox.ru Nuclei of ovarian pseudonurse cells from the mutant strain of Drosophila melanogaster otu 11 are suitable for mapping the attachment of chromosomes to the nuclear envelope (NE). Loci in contact with the NE included region 20CD of the X chromosome, region 41 of chromosome 2, the proximal end of region 81 of chromosome 3, and region 101 of chromosome 4. In situ hybridization revealed that all 4 regions contained sequences homologous to clone λ20pl.4. DNA of clone λ20pl.4 was previously found to bind specifically to purified D. melanogaster lamins. These results suggest that specific DNA sequences are involved in attachment of chromosomes to NE in vivo.