The presence and localization of NADPH-diaphorase in the cerebral ganglion of the shore crab
Hemigrapsus sanguineus was investigated with histochemical and electron histochemical methods. The reactivity of this enzyme
was found in the deutrocerebrum, mainly in neuropils of olfactory lobes, the lateral antennular neuropil, a laterodorsal group of
cells, and in the oculomotor nerve nucleus. Ultrastructural localization of the enzyme was detected in neurons on the perinuclear
membrane, and in membranes of endoplasmic reticulum, in mitochondria and cytosol. The enzyme was found in axons of the antennular
nerve, and in terminals of receptor axons in the glomerulus. The obtained data testify to participation of NO in perception and
processing of the olfactory information.
Key words: NADPH-diaphorase, nitric oxide, brain, Crustacea
