A COMBINED APPROACH TO MAPPING THE PROXIMAL BORDER OF THE RIGHT ARM OF PO-LYTENE CHROMOSOME 2 DROSOPHILA
MELANOGASTER OTU 11
T. V. Bojkova,1 I. V. Sharakhov,2 S. A. Kopyl,1
E. Volkova,2 P. A. Fisher,3 V. A. Rogachev,1 T. E. Sebeleva,1 S. S. Bogachev
1
1 Institute of Cytology and Genetics, Siberian Branch of RAS, Novosibirsk,
2 Institute of Biology and Biophysics, Tomsk State University, Russia, and 3 Department of Pharmacological
Sciences,
University of New York at Stony Brook, Stony Brook, USA;
e-mail: owk_nsk@hotbox.ru
A combined approach based on cytological observations in situ hybridization, and qualitative Southern-blot analyses were
used to localize the proximal border of the right arm of polytene chromosome 2 in Drosophila melanogaster otu 11 strain.
A genetically functional chromosome 2 is bounded by "deletions" C', C, D, B, A and ms2-10. Using in situ hybridization in conjunction
with comparative quantitative Southern-blot hybridization to deletions in centromeric heterochromatin, DNA of specific centromeric clone
λ20pl.4 was localized with respect to "deletions" and on otu 11 polytene chromosomes. Comparison of hybridization sites of
λ20pl.4 on polytene chromosomes, and its amount in mutant lines of D. nielanogaster carrying known
"deletions" in the centromeric heterochromatin enabled us to localize the proximal border of the right arm of chromosome 2 in D.
nielanogaster otu 11 strain between the 39/40 region and hybridization site of the λ20pl.4 DNA fragment.
Key words: centromeric, heterochromatin, functional centromere, Drosophila melanogaster otu 11, DNA λ20p1.4
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