LECTIN AND MITOTIC ACTIVITIES IN ROOT MERISTEMS OF WINTER WHEAT UNDER ORYZALIN
EFFECT
Yu. Yu. Chulkova, L. D. Garaeva, O. A. Timofeeva, L. P. Khokhlova
Department of Plant and Biotechnology, Kazan' State University, Russia;
e-mail: Olga.Timofeeva@ksu.ru
The effect of oryzalin, a microtubule polymerization inhibitor (10 ìÌ), on lectin and mitotic activities (mitotic index
and duration of mitotic phases) was studied in unhardened (23 °C) and hardened (7 days, 2-3 °C) winter wheat seedlings. Three wheat
cultivars differing in their frost tolerance were compared. Oryzalin treatment (3 h) decreased activity of soluble lectins, increased
activity of cell wall lectin mitotic index. Under these conditions, prolongation of anaphases and disappearance of telophases were
detected. Plant hardening reduced the sensitivity of cell wall lectins and mitotic activity to the cytoskeleton inhibitor due,
presumably, to the appearance of cold-stable microtubules. Plant growing and hardening with oryzalin stopped mitoses and caused the
appearance of polyploid cells and cells with micronuclei. These abnormalities were preserved after hardening. The results obtained
demonstrate an important role of microtubules in adaptation of plants to low temperature.
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