DETECTION AND IDENTIFICATION OF A TUMOR-ASSOCIATED HETEROORGANIC ANTIGEN OF ZAJDELA HEPATOMA IN NON-HISTONE
PROTEINS OF CHROMATIN
N. P. Teryukova,1 P. A. Ivashkina, O. A. Mirgorodskaya, V. A. Ivanov
Institute of Cytology RAS, St. Petersburg, Russia;
1 e-mail: iva@mail.cytspb.tssi.ru
By polyacrylamide gel electrophoresis, a phosphoprotein with mol. weight of 42 kDa was detected in non-histone
proteins (NHP) of chromatin of Zajdela ascitic hepatoma cells eluted from phosphocellulose with 0.4-0.5 M NaCl. A protein of the
same mol. weight is present in narrow fractions of rat kidney chromatin, but is absent in rat liver. It is suggested that the
revealed protein corresponds to the tumor-associated heteroorganic NHP antigen detected earlier in NHP chromatin of rat tumor
cells. By MALDI mass spectrometry, this phosphoprotein was identified as ERK2/mitogen-activated protein kinase.
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