THE INFLUENCE OF NITRIC OXIDE INDUCTOR (NaNO2) ON THE SENSITIVITY
OF CHINESE HAMSTER CELLS TO HYPERTHERMIA
O. I. Bolshakova, A. G. Sverdlov, S. I. Timoshenko,
N. G. Nikanorova, S. A. Grachev
St. Petersburg Nuclear Physics Institute RAS, Gatchina, Leningrad District;
Formerly we reported the reduction of sensitivity to gamma- and UV-radiation in Chinese hamster cells of line
90 under the influence of nitric oxide inductor (NaNO2). Of interest was to learn if it possible to reduce the
influence of hyperthermia on cells by means of NO inductor. A 1 h long incubation with this NO donor demonstrated an increased
survival of cells heated up to 45 °C, and a decreased frequency of chromosomal aberrations in these. Employment of cycloheximide
(CHE), an inhibitor of protein synthesis, and administration of CHE together with nitric oxide donor (NaNO2), equally
increased the cell survival at hyperthermia. These and relevant literature data suggest that the demonstrated effect of exogenous
NO may be associated with HSP70 protein. The noticed decrease in the number of chromosomal aberrations in heated cells under the
influence of NO donor may play an important role in its modifying effect on cells at hyperthermia.
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