THE LACK OF CYCLIN-DEPENDENT PHOSPHOPROTEIN KINASE Pho85p LEADS TO DEFECTS IN MITOCHONDRIAL NUCLEOID
TRANSMISSION IN YEAST SACCHAROMYCES CEREVISIAE
E. V. Sambuk,1 A. Yu. Fisikova, K. V. Zakharova, A. M. Smirnov, M. V. Padkina
Biological Research Institute, St. Petersburg State University, St. Petersburg,
Russia;
1 e-mail: sambuk@mail.ru
Cyclin-dependent phosphoprotein kinases (CDK) play essential role in the regulation of the progression through
different phases of the cell cycle, being also involved in metabolic regulation and in actin cytoskeleton organization. The
Saccharomyces cerevisiae CDK Pho85p is associated with 10 different cyclins and phosphorylates different substrates,
among these transcription factors Pho4p and Gcn4p, and a protein involved in actin polymerization being a homologue of
amphiphysin 1 of mammalian Rvsl67p. It is known that mutations in the PHO85 gene have a multiple pleotropic effects, one of
these effects being a rapid accumulation of mitochondrial [rho-] mutations observed on the background of gene
PHO85 inactivation. In this study it was shown that the appearance of [rho-] clones is a result of some defects
in mitochondrial nucleoid transmission from mother to bud cells.
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