THE INFLUENCE OF IMMOBILIZED FIBRONECTIN ON KARYOTYPIC VARIABILITY OF THE INDIAN MUNTJAC SKIN FIBROBLAST CELL
SUBLINE
G. G. Poljanskaya, T. S. Goryachaya, G. P. Pinaev
Institute of Cytology RAS, St. Petersburg, Russia;
e-mail: poljansk@mail.cytspb.rssi.ru
The numerical and structural karyotypic variability has been investigated in the Indian muntjac skin fibroblast
cell subline MT on cultivating cells on the fibronectin-coated surface. In cell subline MT, cultivated on the fibronectin-coated
surface for 1 and 2 days, the character of cell distribution for the chromosome number did not change. In 3, 4 and 8 days, the
character of cell distribution for the chromosome number changed. These changes involve a significant decrease in frequency of
cells with modal numbers of chromosomes, and an increase in frequency of cells with lower chromosome numbers. Many new additional
structural variants of the karyotype (SVK) appear. The observed alterations seem to be due to both disturbances of mitotic
apparatus and selection of SVK, which are more advantageous to changed culture conditions of the cell population. Detachment of
cells from the fibronectin-coated surface, followed by a 1 day cultivation on a hydrophilic surface, commonly used for routine
cell cultivation, does not restore the control cell distribution for the chromosome number, but cultivation in these conditions
for 5 days restore control distribution. The frequency of chromosomal aberrations on cultivation on the fibronectin-coated
surface for 3 and 4 days significantly increases, mainly at the expence of dicentrics (telomeric association). On prolongating
the time of cultivation up to 8 days on the fibronectin-coated surface the frequency of chromosomal aberrations approaches the
control value. Structural instability of chromosomes at cultivation on the fibronectin-coated surface demonstrates nonspecific
reaction of "markerless" cell lines to unfavourable factors of the environment. We discuss possible reasons of differences in the
character of karyotypic variability in cell lines of the Indian muntjac skin fibroblasts on cultivating on laminin and
fibronectin.
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