DISTRIBUTION OF SATELLITE DNA TkS1 IN GENOMES OF SALAMANDERS (SALAMANDRIDAE)
S. N. Litvinchuk,1 O. S. Lashina, V. I. Kazakov
Institute of Cytology RAS, St. Petersburg, Russia;
1 e-mail: slitvinchuk@yahoo.com
Genomes of 22 species and subspecies of salamandrids (Triturus, Cynops, Neurergus, Notophthalmus, Pachytriton,
and Pleurodeles) were studied. Satellite TkS1 (32-33 bp) was found in all examined species, both in direct and reversed orientations.
The use of single-primer PCR, which are homologous to the most conservative part of satellite TkS1, allowed us to reveal DNA fragments,
flanked by single copies (or small tandem repeats) of satellites TkSl having various (5' and 3') orientation. Such fragments were observed in
Pleurodeles walti, Triturus a. alpestris, T. vulgaris lantzi, T. v. vulgaris, Neurergus crocatus and T. vulgaris graecus. The length
of amplified DNA fragments in three subspecies of T. vulgaris differed. This might be connected with unequal amounts and different
distribution of 5' and 3' copies of satellite TkS1 in their genomes. Patterns of DNA amplification in Triturus a. alpestris and
Neurergus crocatus were quite similar. Only two species (Triturus a. alpestrisand T. v. vulgaris) had approximately
equal amounts and similar distribution of 5' and 3' copies of satellite TkS1 in their genomes.
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