PECULIARITIES OF SPONGE HALISARCA DUJARDINI (DEMOSPONGIAE, HALISARCIDA) LARVAL CELLS.
II. SOME CELL TYPES MARKED BY SPECIFIC ANTIBODIES
Yu. I. Mukhina, O. I. Podgornaya, S. M. Efremova
St. Petersburg State University and Institute of Cytology RAS, St. Petersburg;
e-mail: sophia.efremova@pobox.spbu.ru
The recent morphological and experimental data concerning the involvement of flagellated cells in sponge larvae are
contradictory and testify to or against the germinal layers inversion. A study of morphogenetic processes in sponges, in particular larval
metamorphosis, is complicated by dificulties in identification and succession of certain cell types. It is possible to trace the destiny of
flagellated and other larval cells by marking them with antibodies (AB) specified for each cell type. We separated larval and adult sponge
cells ofHalisarca dujar-dini in percoll density gradient and obtained polyclonal AB for the majority of these cell types. The protein pattern
of larval flagellated cells differed significantly from that of other cell types. The major proteins of flagellated, collencyte-like and spherulous
cells were used to raise the corresponding AB. Immunoblot showed all AB to be specific for certain proteins and suitable for
immunofluorescence. The AB for flagellated cells reacted with the apical cytoplasm, but not with the flagellum, the AB for major protein
of collencyte-like cells stained cytoplasm granules. The AB for spherulous cells of the adult sponge reacted with larval spherulous cells
supposed to be of maternal origin. So, the method of cell marking with specific polyclonal AB can facilitate analysis of the layers inversion
problem, as well as elucidate the degree of cell differentiation in larvae, their conformity to cells of the adult sponge or their provisional
destiny.
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