THE INFLUENCE OF GUANINE NUCLEOTIDES AND PROTEIN KINASE C ON Ca2+ FROM
INTRACELLULAR STORES OF PIGS OOCYTES STIMULATED BY THEOPHYLLINE AND dbcAMP
V. Yu. Denisenko,1 T. I. Kuzmina
All-Russian Research Institute of Farm Animal Genetics and Breeding, 189620 St. Petersburg-Pushkin,
Russia;
1 e-mail: vitald@fromru.com
Effect of guanine nucleotides and protein kinase C on Ca2+ exit from intracellular stores of pig oocytes,
stimulated by theophylline and dbcAMP, was investigated using fluorescent dye chlortetracyclme. Effect of cAMP on Ca2+
exit from intracellular stores of pig oocytes was not associated with activation of protein kinase C. In calcium-free medium, cAMP does
not stimulate Ca2+ exit from intracellular stores of pig oocytes treated with GDP. In the presence of GDP, inhibition of protein
kinase C activates Ca2+ exit from intracellular stores of pig oocytes on the action of c AMP. These data suggest the
existence of different effects of guanine nucleotides on Ca2+ exit from intracellular stores of pig oocytes stimulated by cAMP.
Key words: guanine nucleotids, protein kinase C, theophylline, dbcAMP, calcium, oocyte
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