SMALL STABLE RNA LEVEL DEPENDS ON THE PHYSIOLOGICAL STATE OF THE CELL
T. V. Nikitina, 1 * N. Y. Nazarova, 3 N. D. Aksenov, 2
L. I. Tishchenko, 1 P. Tuohimaa 3 V. M. Sedova 2
1 Department of Biochemistry, St. Petersburg State University;
2 Institute of Cytology RAS, St.-Petersburg, Russia, and 3 Medical School, University of Tampere, Finland;
* e-mail: tanik@tn3532.spb.edu
The level of 5S rRNA and tRNAiÌåt1 synthesized by RNA polymerase III was investigated in human
epidermoid carcinoma cells A431 at different physiological states: low and high proliferation and apoptosis. The real-time RT-PCR
method using SYBR Green I was applied to measure certain RNA species in total cellular RNA. The share of 5S rRNA was practically
the same in slowly and actively proliferating A431 cells, but increased about 2.5-fold in apoptotic cells. The share of initiator
tRNAiÌåt1 in actively proliferating and apoptotic cells was 1.5-2.0 times higher than in slowly proliferating cells.
Our results suggest a possible existence of special mechanisms regulating RNA polymerase Ill-directed transcription from
different type promoters in accordance with the physiological state of the cell.
Key words: Class III transcription, small stable RNA; real-time polymerase chain reaction, cell
proliferation, apoptosis
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